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KMID : 0381120120340050575
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Genes and Genomics
2012 Volume.34 No. 5 p.575 ~ p.579
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CACTA transposon-derived Ti-SCARs for cultivar fingerprinting in rapeseed
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Lee Sung-Il
Park Kyong-Cheul
Ha Man-Woong
Kim Kwang-Soo
Jang Young-Seok
Kim Nam-Soo
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Abstract
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Transposon display (TD) is a technique for analyzing polymorphic insertions and excisions of transposable elements. We used the CACTA transposon to develop cultivar-specific transposon insertion-sequence characterized amplified regions (Ti-SCARs) for rapeseed (Brassica napus). Using 16 combinations of TD primers, we detected 19 cultivar-specific fragments among six rapeseed cultivars. Of the 16 primer pairs, 12 successfully amplified targets in B. napus and six amplified novel, cultivar-specific markers. Cultivar-specific markers can be used for cultivar fingerprinting and marker-assisted selection in the rapeseed breeding program. Moreover, because Ti-SCARs are based on transposon insertions, Ti-SCAR markers may be used in reverse genetic techniques for isolating novel genes in plants.
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KEYWORD
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Transposon display (TD), Ti-SCAR, CACTA, Brassica napus
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